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HI 83746

Photometer for the Determination of Concentration of Reducing Sugars


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A critical parameter in the winemaking process

The determination of concentration of reducing sugars (RS) is one of the most important parameters that need to be measured during the wine making process. Following the increase of RS during maturation of grapes can help decide when to start harvest. Having the highest possible sugar content is important because this is the main parameter that defines the commercial value of grapes. During the alcoholic fermentation instead, the decrease of sugars can be followed to decide when fermentation is completed, or allows the taking of corrective actions if the content of RS is too low to obtain the desired alcohol degree or sweetness.

The predominant RS in grape products are glucose and fructose (hexoses). After reaction with excess alkaline cupric tartrate (Fehling reagents), the RS content can be determined colorimetrically. The Fehling method is not an exact determination but an index of the reducing sugar concentration, because the reaction depends upon the amount and kind of RS present. When the reducing sugar content is known at the beginning of fermentation, the potential alcohol degree can be estimated multiplying the sugar concentration (in g/L) by 0.06. Phenols interfere in the Fehling reaction and therefore red wine must be colorized prior to analysis. Wine also contains non-fermentable reducing sugars like pentose which will also be ­­analyzed by this method.

Order Information:
HI 83746-01 (115V) and HI 83746-02 (230V) is supplied with glass cuvettes and caps (4), reagents for about 20 tests (HI 83746A-0 and HI 83746B-0), HI 93703-59 Charcoal, 200 µL automatic Pipette with two plastic tips, 1000 µL automatic pipette with plastic tips (2), instruction sheet for automatic pipette, spoon, funnel, filter paper (25), cuvette cleaning cloth, 12 VDC adapter, batteries, instructions and rugged carrying case.

  • Specifications
  • Accessories
  • Downloads
Range   0.00 to 50.00 g/L
Resolution   0.25 g/L
Accuracy @25°C   ± 0.50 g/L ±5% of reading
Light Source   tungsten lamp
Light Detector   silicon photocell with narrow band interference filter @ 610 nm
Method   Fehling
Environment   0 to 50°C; RH max 95% non-condensing
Battery Type   1.5V AA batteries (4)/ 12 VDC adapter
Auto-off   after 15 minutes of non-use
Dimensions   224 x 87 x 77 mm (8.7 x 3.3 x 3.1")
Weight   512 g (17.6 oz.)
Accessories
HI 731318 Cuvette Cleaning Cloth
HI 731321 Glass Cuvette Set
HI 731350 Plastic Tip Set for 200 µL Graduated Pipette
HI 731341 1000 µL Graduated Pipette
HI 731351 Plastic Tip Set for 1000 µL Graduated Pipette
HI 740142P Graduated Syringe Set (10), 1 mL
HI 740144P Pipette (Tip) for Dosing Syringes
HI 740216 Test Tube Cooling Rack
HI 740217 Lab Safety Shield
HI 740232 Filter Paper Set, Type 1
HI 839800 COD Test Tube Heater
with 25 Vial Capacity
HI 740027P 1.5V AA Battery Set
Reagents & Standards
HI 83746-20 Reagents for Reducing Sugar Analysis
HI 93703-59 Activated Charcoal

Manual: (Download)

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Product Accessories

Accessories
HI 731318
Cuvette Cleaning Cloth
HI 731321
Glass Cuvette Set
HI 731350
Plastic Tip Set for 200 µL Graduated Pipette
HI 731341
1000 µL Graduated Pipette
HI 731351
Plastic Tip Set for 1000 µL Graduated Pipette

Product Manuals
  Manual: Download





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